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The reaction mixture was poured into the dialysis membrane (Molecular weight cutoff 12 kDa) and dialyzed for 3 days in deionized water, the excess EDC.HCl, NHS, and unreacted proline were removed, then the CS-g-P were freeze-dried at -40 C and kept for further reaction

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Loss of epithelial barrier integrity was accompanied by considerable ZZW-115-induced cell detachment as visualized by light microscopy (Fig

The intestinal crypts were dissociated from the tissue by vigorous shaking