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NOX4 has also been confirmed to be a key regulator of the increased expression of proinflammatory mediators in vascular smooth muscle cells (Lozhkin et al

Cell lines stably expressing cytosolic GFP and surface LaccID were achieved by lentivirus infection, followed by FACS sorting

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Contrarily, CD11b upregulation in Munc13-4-knockout (KO) neutrophils was normal in response to the chemotactic peptide fMLF, a physiological stimulus that signals from plasma membrane receptors, further supporting that CD11b plasma membrane upregulation in response to CpG depends on TLR9 activation at endosomes 16

Ongoing investigation into their metabolomic changes will help clarify its potential in supporting metabolic health and inform future dietary guidelines

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