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[20] PCR was performed in a 0.1 mL tube containing 2 L cDNA, 1 L (10 pmol) rat forward primer and reverse primer, 2 L dNTP (1.25 mM each nucleotide), 5 L SybrGreen, and 1L dH 2 O

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Restoring vascular integrity using synthetic analogues of natural antimicrobial peptides may constitute a new therapeutic direction in patients with sepsis

doi: 10.1126/science.abh1654 100 LoweJTaveira-da-SilvaRHilrio-SouzaE

Second, this method lets you see test sample with and without the iodine, which helps remove any uncertainty about whether it has changed color