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Acetic acid solutions may be necessary for certain peptides with poor water solubility

Membranes were blocked with 5% nonfat milk at room temperature for 1 h

2.1.3.4 Decision triggers for chemical conjugation selection Chemical conjugation becomes the primary engineering option when the cargo is non-proteinaceous (e.g., synthetic glycans, lipids, nucleic acids) or when genetic fusion/modular assembly fails to yield structurally stable particles