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The main challenge in immunotherapy lies in converting cold tumors, which are resistant to the immune response, into hot tumors, which are more likely to respond to ICIs [136]

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Plasma glutathione peroxidase concentrations were higher in supplemented animals, with the interaction between treatment and time, where higher concentrations were observed at 40 days of experiment for supplemented calves compared to control

All media were supplemented with 10% heat-inactivated fetal calf serum (BioWest, Riverside, MO) and cells were kept at 37 C and 5% CO 2 in a humidified atmosphere