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Next, beads were washed three times with TEV protease cleavage buffer, and then the recombinant protein was eluted from the resin by incubation overnight at 4 C with 10 g/mL TEV protease to cleave off the desired protein from the GST tag, which was still bound to the GSTSepharose resin after the overnight cleavage reaction

10.1515/chem-2022-0235 221 YuT.XingY.GaoQ.WangD.ChenH.WangH.et al (2023)

Environmental Chemistry Letters , 17 , 849865

The primers may also contain a restriction site and additional bases to permit "in-frame" cloning of the insert into an appropriate expression vector, using the restriction sites present on the primers

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