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NNMT inhibition increases intracellular concentrations of NAD + and SAM in differentiated adipocytes Figure 3A outlines the major elements of the mammalian NAD+ salvage pathway using NA as the starting substrate.[27] Since the NNMT inhibitor 5-amino-1MQ significantly reduced intracellular 1-MNA concentrations (Figures 2B and 2C), we hypothesized that NNMT inhibition in adipocytes would increase intracellular concentrations of the co-substrates NA and SAM and shunt more NA into the NAD + salvage cycle

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Biohacker Protocol (experimental) Dose: 0.25 mg (= 10 clicks) Duration: Continuous Frequency: Twice daily Cycle Interval: No fixed interval / take 1 week off every 8 weeks Goal / Description: Micro-dosing approach focusing on steady fat metabolism

Sterile water or saline degrades the peptide much faster and creates contamination risk

After reconstitution: Store at 28 C , protect from light, use aliquots to reduce handling, and avoid repeated temperature cycling

And heres where it gets more interesting NNMT is hyperactive in conditions like obesity & type 2 diabetes, Alzheimers & Parkinsons disease, aging in general