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Neurons were blocked in 500 L of blocking buffer (1% Triton X-100, 10% cosmic calf serum in 1X PBS) for 1 h, then incubated in 300 L of primary antibody diluted in blocking buffer overnight at 4 C (See Table S4 for antibody concentrations)

Physical and chemical stability of curcumin in aqueous solutions and emulsions: impact of pH, temperature, and molecular environment

These amplicon data are processed through pipelines like QIIME 2, LotuS2, or ITSx, which include quality filtering, chimera removal, OTU/ASV clustering, and taxonomic assignment using curated databases such as SILVA for 18S and UNITE for ITS (Gao et al., 2021

The progress of MSNs based protein therapy for various applications including intracellular delivery, extracellular delivery, antibacterial proteins delivery, enzyme mobilization and catalysis will be highlighted

(2023) 12:e85289