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Proteins were visualized via chemiluminescence using the ECL Advance Western Blotting Detection Kit (Amersham Biosciences), and images were captured and analyzed by densitometry using a Kodak ID Version 3.6 Image Analyzer (Kodak, NY, United States)

Although the precise receptor for SHBG has not been identified to our knowledge, SHBG has been reported to bind to G protein-coupled receptor 6A [194], which is proposed to regulate energy metabolism in insulin-sensitive tissues as well as stimulate the release of glucagon-like peptide-1 and adiponectin, key regulators of glucose homeostasis [195]