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The organic layer was reconstituted in chloroform:methanol (2:1 v/v, 200 l and diluted 1:10 with 2:1:1 isopropanol:acetonitrile:water, with the addition of an internal standard mix composed of isotopically labelled compounds from major lipid classes

In conclusion, argyria typically has an occupational or iatrogenic etiology

Intentional misuse by deliberately concentrating and inhaling contents can be harmful or fatal

Given the lack of clinical trials to validate the efficacy of natural flavonoids in CNS disease patients and the challenges in translating animal data to clinical settings, this study focuses on the application details of natural flavonoids in CNS disease animal models, including administration routes, dosages, and treatment durations

Diagnostic biomarkers thus serve to exclude such cases, preventing confounding and improving the interpretability of trial results

This is not specific to the poplar isoform as this particularity is found in several plant orthologs, including the petunia AN9 protein for example