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The fly homogenates were incubated in Griess reagent at room temperature for 20 min, and the absorbance measured at 550 nm

(They decrease the amount of mRNA produced from the gene that codes for these components.)

If you need to use small amounts, aliquot the peptide solution into smaller vials before freezing

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Talib M, Rachdi M, Papazova A, Nicolis H (2024) The role of dietary patterns and nutritional supplements in the management of mental disorders in children and adolescents: an umbrella review of meta-analyses: Le rle des habitudes alimentaires et des supplments nutritionnels dans la prise en charge des troubles mentaux chez les enfants et les adolescents: une mta-revue de mta-analyses