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To confirm that GCL activity wholly accounted for ROS down-modulation, we expressed (Fig

In addition, baicalin also reduced KLF6, a fibrogenic nuclear protein that is directly targeted by miR122 (Zhang et al., 2016)

Extracted proteins were denatured in 100 L of 6 M urea, reduced with 10 mM dithiothreitol (DTT) at room temperature for 60 min, and alkylated with 15 mM iodoacetamide in the dark for 60 min

doi: 10.1016/j.heliyon.2021.e06734 153 NguyenNQNguyenMTNguyenVTLeVMTrieuLHLeXTet al

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27 The preventive antioxidant system comprises molecules that inhibit oxidative damage by suppressing the supply of ROS