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Finally, induction of the expression and activity of GSTs and other phase II detoxification/antioxidant enzymes by isothiocyanates is an important defense mechanism against oxidative stress and damage associated with the development of diseases like cancer and cardiovascular disease (22)

Both the essential oil and trans-anethole (the main component) exhibited strong inhibitory effect against all test fungi

As the Figure 7 illustrates, the group treated with the BHE extract showed a TNF level of 8.680.33 pg.mg of protein 1 , which is less than half of the value corresponding to the control group: 19.981.61 pg.mg of protein 1

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Processing of the brains After assessment of the motor performance, rats were euthanized by injection of ketamine and xylazine (80 mg/kg: 12 mg/kg, i.p.)

Plates were washed in running water, air-dried and the stain dissolved in 10% acetic acid on a shaker before absorbance at 595 nm