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Proteins were eluted by incubation with 25 L of 100 g/ml 3xFLAG peptide (Sigma) in 0.05% RapiGest (Waters Corp) in IP buffer on an end-over-end tube rotator at 10 rpm at 4 C overnight

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The main reported result was a large difference in mortality among patients with ALF compared to acute liver injury, both defined according to the United States Acute Liver Failure Study Group criteria

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In cancer, ketones exhibit diverse effects, either promoting or inhibiting cancer cell proliferation

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