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The absorbance at 575 nm was measured on a Synergy H1 microplate reader (BioTek, VT, USA)

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After topical administration, chitosan nanoparticles, as potent penetration enhancers for dual-drug delivery, present synergistic anti-reactive-oxygen-species (ROS), anti-inflammatory, and antimicrobial effects by significantly prolonging drug retention time, maintaining sustained drug release, and improving bioavailability in the choroid and retina [39,40,41]