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Mobile phase A consisted of 0.1 % (v/v) formic acid and mobile phase B consisted of 90% (v/v) acetonitrile and 0.1% (v/v) formic acid

doi: 10.1016/j.jmb.2010.11.018

After a centrifugation at 12,000 g for 15min at 4C, the supernatant was collected, and the protein concentration was measured using a bicinchoninic acid assay

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Abstract The presence of auto-antibodies that target synaptic machinery proteins was documented recently in immune-mediated cerebellar ataxias