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One of these amino acids, methionine, cannot be synthesized by our bodies and therefore has to be supplied by the diet

It skips the digestion problem

Green tea extract, standardized to 55% EGCG, is included for its antioxidant properties and its role in supporting metabolism and weight management

After liquid-nitrogen freeze-thaw, a Dounce homogenizer, 1mL (Active Motif) was used to crush the cardiac tissue, with the addition of 250 uL of lysis buffer (20mM HEPES, 0.01% SDS ( v/v ), pH 7.6, 1x PhosSTOP phosphatase inhibitor per 50mL and 1x Pierce Protease Inhibitor Mini Tablets per 10mL), before proceeding with probe sonication as described in Caenorhabditis elegans MS sample preparation

doi: 10.1158/1535-7163.MCT-08-0198 36 Holmquist-MengelbierLFredlundELfstedtTNogueraRNavarroSNilssonHet al

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