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Protein in sample A and B was removed by precipitating with 200 L of cold ACN (containing 1 g/mL carbachol, the internal standard, IS) and centrifuging at 16,900 g for 20 min

doi: 10.3390/antiox9070624 55 ChenNCYangFCapecciLMGuZSchaferAIDuranteWet al

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Pathological hallmarks of PD such as accumulation of abnormal -synuclein [3] are detected in the enteric nervous system of PD patients before disease development and in individuals at high risk of developing PD such as those suffering from idiopathic REM (rapid eye movement) sleep behaviour disorder (iRBD) [4,5,6,7]