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Two to three-month-old of both sexes SCD mice were treated with 50 mg/kg DMF (#242926, Sigma) every other day for 4 weeks

A practical bench rule helps here: if the vial still shows undissolved material, give it time before touching it again

Vitamin B12 is measured in micrograms (mcg) , with a standard dose of 1,000 mcg per injection

StatPearls [Internet], 2024 Jan Avinoam Shiran, Eric Remer, Ihab Asmer, Basheer Karkabi, Eran Zittan, Aliza Cassel, Mira Barak, Orit Rozenberg, Khaled Karkabi, Moshe Y Flugelman

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In addition, metallothionein 3 (MT3) can extract Cu 2 + from amyloid- or -synuclein complexes and facilitate its reduction to Cu + , a property attributed to its flexible N-terminal -domain containing a unique threonine insertion at position 5 that distinguishes MT3 from other metallothionein isoforms 195,196