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Evidence for a reversible rChTg-GSSG complex Due to the facile aggregation of the reduced enzyme at neutral pH values into insoluble polymers 19 , all fluorescence experiments were performed at pH 5.0 observing the quenching of the intrinsic fluorescence after suitable addition of GSSG

Unadjusted p-values were derived from the two-sided Wilcoxon rank-sum test.The box plots centre line represents the median, with the lower and upper boundaries indicating the first and third quartiles, and whiskers extend 1.5 times the interquartile range from the lower and upper quantiles