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PMID: 42383012 iScience Dynamic regulation of P-TEFb by 7SK snRNP is integral to the DNA damage response to regulate chemotherapy sensitivity

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Methods: In this study, 24 HBeAg+ pregnant women with chronic HBV infection were enrolled and followed up from pregnancy to postpartum, and 12 HBeAg+ non-pregnant women in the stage of immunotolerance (IT) were included as control

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Fine-tuning GPCR-mediated neuromodulation by biasing signaling through different G protein subunits

Tumour cells were grown as monolayers in high-glucose (4500 mg/L) DMEM supplemented with 2 mM GlutaMAX supplement, 50 IU/mL penicillin, 50 mg/mL streptomycin and 10% foetal bovine serum (FBS), whereas they were cultured as mammospheres in MammoCult basal medium supplemented with 10% MammoCult proliferation supplement (human serum), 4 g/mL heparin and 0.48 g/mL hydrocortisone for 10 days (STEMCELL technologies, Vancouver, Canada)