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Goat anti-mouse IgG or anti-rabbit IgG (Boster, Wuhan, China) conjugated to HRP was used as the secondary antibody

Copper toxicology, oxidative stress and inflammation using zebrafish as experimental model

Think of it as pressing the pause button on any potential bacterial contaminants that might be introduced into the vial after the factory seal is broken

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I hope this was just a one off, but when I gingerly cut around the edge lining and opened the seal, met with a very bad odor and saw that the underside of the seal looked damaged