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As a result, attaining satisfactory yield and purity depends on highly selective and efficient capture of these proteins from the crude cell lysates

Ronald B

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Eid et al

The expression plasmid pCGPx3M encoding the human GPx3 mutant was constructed by subcloning the Nde I/ Hin d III fragment from pUC57-GPx3M into the Nde I/ Hin d III sites of the pCold I vector (TaKaRa)

On day 6, cells were treated with DMSO or the corresponding SCLA for 2 h, followed by the indicated alkyne-modified probes for 2 h, washed with ice-cold PBS three times, cell pellets collected by scraping and stored at 80 C until further processing