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Binding of FITC-labelled PVL to platelets and neutrophils LukS-PV and LukF-PV were labelled with FITC as described 68

Among the various compounds synthesized, the alkyl-based PROTAC 73 stands out, capable of inducing in vitro a selective and potent proteasome-dependent degradation of the target protein (DC 50 value of 214 nM), resulting in a favorable antiproliferative effect (IC 50 value of 1.3 M) 128

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To further support the in vivo findings, we conducted in vitro coculture experiments that mimic the microenvironment of TLS (Supplemental Figure 10A)

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