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When it activates that receptor on pituitary cells, calcium is mobilized inside the cell and a short, controlled pulse of growth hormone follows

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Callis offered Perry a spot in the Don Callis Family

However, note that although it is true that complete denaturation abolishes GFP fluorescence, recovery of fluorescence does not perfectly parallel the structure recovery, meaning that even a partial refolding could lead to fluorescence recovery, as demonstrated by urea denaturation at pH 6.2 slightly above the pI of GFP (pH 5.6), which does not appear to lead to full denaturation [27]

Guan Y, Myers CL, Lu R, Lemischka IR, Bult CJ, Troyanskaya OG

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