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While we did not find evidence of a gene or signaling pathway that regulates an LCA-specific response, the LCA-induced biofilm phenomenon may be related in part to the inherent tendency of EcN to self-aggregate during stationary phase in YCFA, as nearly all biofilm-deficient mutants in our transposon mutagenesis screen also displayed abnormal or reduced self-aggregation

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W., Poynter, J

The entire procedure concludes within 30 minutes, so it can be slotted into a busy schedule

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