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The mislocalization of Pf GS was induced by the addition of 250 nM rapamycin (Sigma-Aldrich, R0395) and verified by the translocation of GFP fluorescence from the parasite cytosol to the plasma membrane in live parasites

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The l-EV fraction from FLAG-MIM I-BAR- and Halo7-Rac1-co-expressing cells was serially diluted in the lysis buffer, and the Rac1 amount was measured by ELISA simultaneously with the cell lysates

One may also look at using natural forms of folate, rather than synthetic folic acid

doi: 10.1093/cvr/cvaa302

Therefore, for each pH both buffers were mixed in a 1:1 ratio and the pH of this solution was measured such that the final pH was known