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Reactions were initiated by the addition of 10 reaction buffer (250 mM Tris-HCl pH 7.5, 500 mM NaCl, 50 mM MgCl, 50 mM ATP, 5 mM DTT) and incubated at 37 C for the specified durations

However, excessive CARS can lead to immunoparalysis, making the body susceptible to pathogens and serving as an important basis for secondary postoperative infections (51)

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Supplements can still be effective, especially if you're looking to maintain your B12 levels over time

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