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Transduced cells were selected with 2 g/ml puromycin (Sigma-Aldrich, TR-1003-G).The DNA sequences of these shRNA genes were as follows: sh-NRF2-a forward: 5- CCG GCT TGC ATT AAT TCG GGA TAT ACT CGA GTA TAT CCC GAA TTA ATG CAA GTT TTT G-3 and reverse, 5- AAT TCA AAA ACT TGC ATT AAT TCG GGA TAT ACT CGA GTA TAT CCC GAA TTA ATG CAA G-3, sh-NRF2-b forward: 5-CCG GAG AGC AAG ATT TAG ATC ATT TCT CGA GAA ATG ATC TAA ATC TTG CTC TTT TTT G-3 and reverse, 5- AAT TCA AAA AAG AGC AAG ATT TAG ATC ATT TCT CGA GAA ATG ATC TAA ATC TTG CTC T-3, sh-C forward: 5- CCG GGC AAG CTG ACC CTG AAG TTC ACT CGA GTG AAC TTC AGG GTC AGC TTG CTT TTT G-3 and reverse, 5- AAT TCA AAA AGC AAG CTG ACC CTG AAG TTC ACT CGA GTG AAC TTC AGG GTC AGC TTG C-3

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PMID: 40413364 Drug Des Devel Ther Melatonin Attenuates Sepsis-Induced Acute Lung Injury via Inhibiting Excessive Mitophagy

It's like watering plants: regular, constant attention does better than periodic lushness and nothing for a week

A previous study revealed that genistein can enhance the AMPK signaling pathway and SIRT1 activity through GPER activation, subsequently inhibiting the expression of genes associated with hepatic lipogenesis and further influencing liver metabolic status [169]

All the experiments followed the Guidelines of Ethical Standards for Investigation of Experimental Pain in Animals