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Subsequently, the research group further highlighted that PF effectively attenuates neuroinflammation in the retinal cells of diabetic rats through the inhibition of the JAK2/STAT3 signaling pathway (Zhang et al., 2023d)

The resulting formazan crystals were dissolved in 100 L of dimethyl sulfoxide (DMSO) with gentle shaking for 5 min, and absorbance was subsequently measured at 570 nm using a microplate reader

the reduced dye then undergoes non-enzymic oxidation in air, so the addition of a relatively small amount of methylene blue will effectively deplete NADPH, and would be expected to stimulate any pathway that reduces NADP+ to NADPH

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