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SF3B1 in-vitro binding assays 1 g of purified GST-tagged SF3B1(1463) in 48 l of kinase buffer (20 mM Tris-HCl, pH=8.0, 5 mM MgCl 2 , 2 mM DTT, 2 mM ATP) was incubated with 12 ul flag-tagged CDK11 kinase (immunoprecipitated from HEK293 cells) or with 12 ul of elution buffer (20 mM Tris-HCl (pH=7.5), 150 mM NaCl, 1 mM DTT)

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Furthermore, nanomaterials facilitate the penetration of drugs across ocular barriers, allowing them to reach the target tissues more effectively [186]

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RNA interference (RNAi) has the advantage of enabling disease-modifying drugs, as it can reduce the expression of disease-causing genes in a sequence-specific manner