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For serum samples, 75 L of chloroform:methanol extraction solvent (v:v = 1:2) was added to 20 L of mouse serum, with the exception of the serum from WT, Gclc L-KO, Nrf2 L-KO, and L-DKO mouse serum, for which 20 L of mouse serum was combined with 180 L of chloroform:methanol extraction solvent (v:v = 1:2) containing internal standards at the final concentrations: 5 nM D7-Sphinganine (Avanti Polar Lipids Inc., Cat# 860658), 12.5 nM D3-Deoxysphinganine (Avanti Polar Lipids Inc., Cat# 860474), and SPLASH LIPIDOMIX (1:1000, Avanti Polar Lipids Inc., Cat# 330707)

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However, we question this assertion because age eligibility in a trial is not a clinical outcome, and eligibility may not correlate with improved outcomes

doi:10.1016/j.placenta.2018.05.013 917. Shaarawy M, Al-Sokkary F, Sheba M, Wahba O, Kandil HO, Abdel-Mohsen I

Judah Folkman, a researcher at Children's Hospital at Harvard Medical School in Boston