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Primers for EF1A and IFNG were taken from refs

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Hilmer, Jonathan K

GdmCl-induced chemical unfolding GdmCl-induced denaturation of 0.1 g/l hPrdx6 at pH 7.0 (10 mM TrisHCl buffer) and pH 4.0 (10 mM sodium acetate buffer) was followed by measuring changes in max as a function of GdmCl concentration (0-5 M) at 20 C in Cary Eclipse Fluorescence Spectrophotometer (Agilent Technologies) with an external constant temperature circulator/bath

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However, finding a general biomarker for cognitive impairment is difficult because many biomarkers and mechanisms are involved in different types of cognitive impairment