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The method according to claim 2, wherein the organic solvent in step (a) is at least one solvent selected from the group consisting of acetone, ethanol and methanol

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The exclusion criteria were: patients suffering from lung disease except extensive bronchiectasis, such as cystic fibrosis, and pulmonary fibrosis

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Sample size Previously reported results [12] were adopted in two-way ANOVA sample size calculation using Statistica 13.1 software (Dell Inc., Tulsa, OK, USA)

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