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Glutathione also helps lighten the skin by converting melanin to a lighter color and deactivating tyrosinase, which is the enzyme responsible for catalyzing the production of melanin

2024 Oct 1;46(10):1105774

For cDNA synthesis, 3 g of total RNA was reverse transcribed in a 20L reaction mixture containing 1 L of random oligonucleotide primers, 1 L of 10 mM dNTPs, 4 L of 5X RT buffer, 1 L of 100 mM DTT, 1 L of RNAseOUT TM (40 U/L), 1L of SuperScriptR TM IV reverse transcriptase (200 U/L) (Invitrogen, Waltham, USA), and RNase-free water

Furthermore, analysis of the AppA produced via gel-shift assay showed that the AppA produced by co-expression of Erv1p and DsbC in a wild-type E