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After washing, sections were incubated 30 min with horseradish peroxidase-labelled goat-anti-rabbit (GARPO) or rabbit-anti-goat (RAGPO) secondary antibodies diluted in 1% BSA/PBS + 1% normal human (AB) serum to stop unspecific binding

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After 4 days, the TS2/16 scFv-Fc was captured from the conditioned media with CaptivA HF protein-A affinity resin (Repligen, CA-PRI-0100) and eluted with IgG elution buffer (Thermo Scientific) containing 300 mM NaCl and neutralized with 100 mM Tris pH 8.0 to reach eventually pH 7.0