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These are the active forms of Vitamin B12

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The cell lines were then serum-deprived, seeded at a density of 0.2 or 2105 cells/cm2 in 35-mm dishes (Asahi Techno Glass), 60-mm dishes (Asahi Techno Glass) or 100-mm dishes (Becton Dickinson Labware) in serum-free MCDB-104GK medium supplemented with penicillin (5 g/ml), streptomycin (5 g/ml) and neomycin (10 g/ml) and incubated for 24 h