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Because of the hypothesised abnormalities in oxidative stress and suggested links between initial inflammation, glutamate excitotoxicity and neuronal dysfunction/loss [25], our additional interest was in, GSH, glutamate and glutamine, and total choline as biomarkers of these processes

Recordings were conducted at room temperature in the presence of the GABA A R blocker picrotoxin (0.1 mM) and the Na + channel blocker Tetrodotoxin (0.001 mM)

Sucrose-preference testing of anhedonia The second chronic NAC treatment was terminated on the evening before the test day, and around 9 AM of the test day, animals were single-housed in cages with two water bottles filled with normal drinking water

If fatigue is part of the story, the team can review whether B12 fits the picture or whether broader evaluation would be smarter

doi: 10.1007/s12011-008-8174-z 15

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