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Live intracellular fluorescence stainings CellROX Orange Reagent, for oxidative stress detection (C10443, Invitrogen, Thermo Fisher Scientific), FerroOrange (F374, Dojindo), and BODIPY 581/591 C11 (D3861, Invitrogen, Thermo Fisher Scientific) were used according to the manufacturers instructions

Except for S8 site, most of the hotspots did not coincide with experimental fragment binding sites

By systematically reviewing and synthesizing existing literature, this article aims to provide insights for further investigation into ferroptosis mechanisms in OA and to broaden potential therapeutic targets and approaches for clinical management of ferroptosis-related pathological processes

SGLT2 inhibitors: a novel player in the treatment and prevention of diabetic cardiomyopathy

Chromatins were sonicated to 2001000 bp and immunoprecipitated with antibodies against NRF2, H3K4Me3, and H3K9Ac and determined with gene-specific primers (Supplementary Table 1)

TABLE 3 A typical sign of eryptosis