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Embryos were recovered by standard non-surgical flushing with lactated ringers solution (ICU Medical, San Clemente, CA) supplemented with 1% (v/v) fetal bovine serum on embryonic day 12

Polymerase chain reaction (PCR) amplification was performed in the presence of 2.25 mM MgCl 2 , 0.08 M of each primer, 0.2 mM dNTP, 1.25 U of Taq polymerase (Roche Diagnostics, Mannheim, Germany) and 5 L of each cDNA to a final volume of 50 L

In addition to cerebral embolism as a possible mechanism underlying cognitive decline, AF may also lead to transient or chronic cerebral hypoperfusion (277)

doi: 10.1177/2053369120924166

accumulation of C16-, C18- and C18:1-carnitine

The researchers then used the blunt end of a surgical tool to gently compress the thinned area, recreating a concussion