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In the study by Neelakantan et al

Levels of cellular NAD + were measured using NAD/NADH quantification kits according to the manufacturers instructions (Sigma-Aldrich, MAK037)

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In vitro experiments demonstrated that NNMT overexpression significantly increased Sirt1 protein expression in primary hepatocytes, while NNMT knockdown led to a notable decrease in Sirt1 protein expression

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