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Each strain was grown overnight at 37 C in 2 mL of LB supplemented with 0.5% glucose and 10 g/mL gentamicin, then diluted 1:100 into 100 mL LB supplemented with 10 g/mL gentamicin and grown at 37 C for 30 min

( g ) Representative cryoET slices of gB vesicles from one of at least n = 2 independent, successful productions and purifications of vesicles with either wild type or the final expression construct featuring a C-terminal Twin-Strep-tag

168 As reviewed by Brierley & Kelber, compounds like gingerol, menthol, and eugenol target TRP channels (e.g., TRPA1, TRPM8), which are responsible for pain and motility regulation in the GI tract