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Immunoprecipitation (IP) and WB analysis IP was performed on hepatocyte (which were isolated from three animals and transduced as indicated in the specific figures) lysates using antibodies against LxRxx[S*/T*] and Rxx[S*/T*] phospho-motifs (both Cell Signaling Technology) with Pierce Protein A/G Magnetic Beads according to the manufacturers protocol

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Glutathione is homeostatically controlled, both inside the cell and outside

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It also incorporates, when available, Ontario data and information provided by experts and applicants to Health Quality Ontario