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doi: 10.1089/ars.2012.4640

Digestion-based DNA methylation analysis Percentage DNA methylation was assessed at CpG sites across CpG islands in CYP2R1, CYP27A1, CYP27B1, CYP24A1, and VDR genes using Qiagen EpiTect II methylation enzyme kits and PCR assays at the concentrations and cycle conditions recommended by the manufacturer

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Medical Director, Heart and Vascular Center of Excellence Chief, Division of Cardiovascular Medicine University of Massachusetts Medical School and Memorial Medical Center Mary C

These enzymes function in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione

The connection between VDR-FokI SNP in exon 2 and HT was described years ago by showing that polymorphisms in the 3 region of the VDR gene were linked with HT in the Croatian population and probably affected VDR mRNA expression