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PCA was performed before clustering and the first 20 principal components were used based on the ElbowPlot

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For example, glucocorticoids can suppress the hypothalamic-pituitary-adrenal (HPA) axis, potentially leading to osteoporosis and other adverse effect (Schcke et al., 2002)

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Later he came to believe that vitamin C could prevent cardiovascular disease, cancer, and combat aging7