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(2) changes to GLUT4 traffic in insulin resistance

The cell lysates were centrifuged at 12,000 g for 10 minutes and the supernatant was mixed with the FLAG antibody overnight at 4C on a shaker, before incubation with prewashed protein A/G agarose beads at 25C for 4 hours

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A gradient elution was implemented at a flow rate of 450 l min 1 , where solvent A was water containing 10 mM ammonium acetate (pH 9), and solvent B was a mixture of acetonitrile and water (95:5 v/v) with the same ammonium acetate concentration

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