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Aspartic acid has an acidic side chain with a pKa of 3.86 (pKa 3) and the carboxylic acid pKa is 2.09 (pKa 1): pI = (pKa of carboxyl group + pKa of side chain group) / 2 pI = (2.09 + 3.86) / 2 2.98 Lysine has a basic side chain with a pKa of 10.79 (pKa 3) and the amino pKa is 8.95 (pKa 2): pI = (pKa of amino group + pKa of side chain group) / 2 pI = (8.95 + 10.79) / 2 9.87 The concept of the isoelectric point is critical in understanding protein behavior, especially during techniques like electrophoresis, where proteins can be separated based on their charges at different pH values

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Its antioxidant properties further support barrier function by neutralizing free radicals, as noted in Pickart et al

They are research chemicals and are not approved for human use by regulatory bodies in many places