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One proof-of-concept clinical trial demonstrated that subtotal RFA or MWA may augment the tumor-directed immune response stimulated by these agents to increase the overall response rate and improve median overall survival (OS) in patients with HCC (86)

Before data collection, the crystals were soaked in 25% PEG4000, 25% PEG400, 50 mM MgCl 2 , 100 mM Tris (pH 8.5), and 10% glycerol

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The resultant cell suspension (10 6 cells/mL) was loaded in a poly-L-lysine-coated 96-well culture plate and incubated at 37 C with 5% CO 2 overnight to allow for monocyte adherence