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Emergency medicine journal: EMJ, 20(2), 164

Eyes were either fixed in 4% paraformaldehyde (PFA) for 4 h upon collection for retinal flat mount preparation, or embedded and frozen in OCT compound (Sakura Fintek, Zoeterwoude, The Netherlands) for preparation of 10 m fresh-frozen sections to be used for TUNEL assay, DHE fluorescence staining and NT immunostaining