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Imidazole ketone Erastin (IKE) achieves markedly greater potency and stability through an imidazole ketone scaffold that enables durable target engagement while resisting metabolic clearance (76)

& Blobel, G

Reaction Termination: Add 50 L of Stop Solution to each well

The glutathione (GSH) concentration found in this environment (micromolar) is much lower than that found intracellularly (millimolar) by about a thousand-fold